Genome-scale identification of SARS-CoV-2 and pan-coronavirus host factor networks.

William M SchneiderJoseph M Luna Hans-Heinrich Hoffmann Francisco J Sánchez RiveraAndrew A LealAlison W Ashbrook Jérémie Le Pen Eleftherios Michailidis Inna Ricardo-LaxAvery PeaceAnsgar F StenzelScott W Lowe Margaret R MacDonald Charles M Rice John T Poirier

Published in: bioRxiv : the preprint server for biology (2020)

Genome-wide CRISPR screens for SARS-CoV-2, HCoV-OC43, HCoV-NL63, and HCoV-229E coronavirus host factors.Parallel genome-wide CRISPR screening uncovered host factors and pathways with pan-coronavirus and virus-specific functional roles.Coronaviruses co-opt multiple biological pathways, including glycosaminoglycan biosynthesis, SREBP signaling, and glycosylphosphatidylinositol biosynthesis and anchoring, among others.TMEM41B - a poorly understood factor with roles in autophagy and lipid mobilization - is a critical pan-coronavirus host factor.

Keyphrases

sars cov
genome wide
dna methylation
respiratory syndrome coronavirus
copy number
gene expression
crispr cas
signaling pathway
genome editing
endoplasmic reticulum stress
fatty acid