Anti-Inflammatory and Antioxidant Effects of Leaves and Sheath from Bamboo ( Phyllostacys edulis J. Houz).
Rosa TundisGiuseppina AugimeriAdele VivacquaRosa RomeoVincenzo SicariDaniela BonofiglioMonica Rosa LoizzoPublished in: Antioxidants (Basel, Switzerland) (2023)
Bamboo ( Phyllostacys edulis J. Houz) has become an emerging forest resource of economic and ecological significance with health benefits. Since the beneficial effects of the non-edible parts of bamboo have not been thoroughly explored, we characterized in this study bamboo leaf (BL) and sheath (BS) extracts. The total phenol and flavonoid content (TPC and TFC), antioxidant activity (ABTS, DPPH, FRAP and β-carotene bleaching test) and anti-inflammatory properties were determined. Leaves exhibited a TPC value of 73.92 mg equivalent (eq) gallic acid/g fresh weight (FW) and a TFC value of 56.75 mg eq quercetin/g FW. Ultra-High-Performance Liquid Chromatography (UHPLC) coupled with photo diode array detector (PDA) analysis revealed evidence for the presence of protocatechuic acid, isoorientin, orientin and isovitexin in BL, whereas BS was rich in phenolic acids. Both samples demonstrated a significant ability to scavenge radicals against ABTS ·+ , with an inhibitory concentration of 50% of 3.07 μg/mL for BL and 6.78 μg/mL for BS. At a concentration of 0.1 and 0.2 mg/mL, BS decreased reactive oxygen species production without hampering cell viability in HepG2 liver cells, while at the same concentrations, BL exhibited cytotoxicity in HepG2 cells. In addition, 0.1 and 0.2 mg/mL BS and BL reduced Interleukin-6 and Monocyte Chemoattractant Protein-1 production in human lipopolysaccharide-stimulated THP-1 macrophages, without affecting cell viability. These findings highlight the anti-inflammatory and antioxidant properties of BL and BS, corroborating their different potential applications in the nutraceutical, cosmetic and pharmaceutical industries.
Keyphrases
- anti inflammatory
- reactive oxygen species
- ultra high performance liquid chromatography
- tandem mass spectrometry
- endothelial cells
- oxidative stress
- healthcare
- public health
- climate change
- simultaneous determination
- body mass index
- high resolution
- ms ms
- risk assessment
- human health
- toll like receptor
- induced apoptosis
- dendritic cells
- weight loss
- high throughput
- high resolution mass spectrometry
- nitric oxide
- small molecule
- physical activity
- immune response
- cell proliferation
- lps induced
- weight gain
- induced pluripotent stem cells
- binding protein
- cell death
- pi k akt
- health promotion
- data analysis