Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments.
Glenn Robert MassonJohn E BurkeNatalie G AhnGanesh S AnandChristoph BorchersSébastien BrierGeorge M Bou-AssafJohn R EngenS Walter EnglanderJohan FaberRachel GarlishPatrick R GriffinMichael L GrossMiklos GuttmanYoshitomo HamuroAlbert J R HeckDamian HoudeRoxana E IacobThomas J D JørgensenIgor A KaltashovJudith P KlinmanLars KonermannPetr ManLeland MayneBruce D PascalDana ReichmannMark SkehelJoost SnijderTimothy S StrutzenbergEric S UnderbakkeCornelia WagnerThomas E WalesBenjamin T WaltersDavid D WeisDerek J WilsonPatrick L WintrodeZhongqi ZhangJie ZhengDavid C SchriemerKasper Dyrberg RandPublished in: Nature methods (2019)
Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical technique being increasingly applied to a wide variety of problems. As the HDX-MS community continues to grow, adoption of best practices in data collection, analysis, presentation and interpretation will greatly enhance the accessibility of this technique to nonspecialists. Here we provide recommendations arising from community discussions emerging out of the first International Conference on Hydrogen-Exchange Mass Spectrometry (IC-HDX; 2017). It is meant to represent both a consensus viewpoint and an opportunity to stimulate further additions and refinements as the field advances.
Keyphrases
- mass spectrometry
- liquid chromatography
- mental health
- healthcare
- gas chromatography
- high performance liquid chromatography
- capillary electrophoresis
- clinical practice
- high resolution
- electronic health record
- primary care
- multiple sclerosis
- ms ms
- tandem mass spectrometry
- big data
- visible light
- case report
- machine learning