Exogenous erythropoietin increases hematological status, fat oxidation, and aerobic performance in males following prolonged strenuous training.
Devin J DrummerJulie L McNiffEmily E HowardJess A GwinChristopher T CarriganNancy E MurphyMarques A WilsonJulia MichalakBenjamin J RyanJames P McClungStefan M PasiakosLee M MargolisPublished in: Physiological reports (2024)
This study investigated the effects of EPO on hemoglobin (Hgb) and hematocrit (Hct), time trial (TT) performance, substrate oxidation, and skeletal muscle phenotype throughout 28 days of strenuous exercise. Eight males completed this longitudinal controlled exercise and feeding study using EPO (50 IU/kg body mass) 3×/week for 28 days. Hgb, Hct, and TT performance were assessed PRE and on Days 7, 14, 21, and 27 of EPO. Rested/fasted muscle obtained PRE and POST EPO were analyzed for gene expression, protein signaling, fiber type, and capillarization. Substrate oxidation and glucose turnover were assessed during 90-min of treadmill load carriage (LC; 30% body mass; 55 ± 5% V̇O 2 peak) exercise using indirect calorimetry, and 6-6-[ 2 H 2 ]-glucose PRE and POST. Hgb and Hct increased, and TT performance improved on Days 21 and 27 compared to PRE (p < 0.05). Energy expenditure, fat oxidation, and metabolic clearance rate during LC increased (p < 0.05) from PRE to POST. Myofiber type, protein markers of mitochondrial biogenesis, and capillarization were unchanged PRE to POST. Transcriptional regulation of mitochondrial activity and fat metabolism increased from PRE to POST (p < 0.05). These data indicate EPO administration during 28 days of strenuous exercise can enhance aerobic performance through improved oxygen carrying capacity, whole-body and skeletal muscle fat metabolism.
Keyphrases
- skeletal muscle
- high intensity
- adipose tissue
- gene expression
- hydrogen peroxide
- physical activity
- insulin resistance
- resistance training
- oxidative stress
- clinical trial
- fatty acid
- dna methylation
- amino acid
- randomized controlled trial
- small molecule
- electronic health record
- study protocol
- blood glucose
- cell proliferation
- blood pressure
- type diabetes
- simultaneous determination
- cell cycle arrest
- phase iii
- artificial intelligence
- cross sectional
- high resolution
- glycemic control
- weight loss
- pi k akt
- solid phase extraction
- virtual reality
- liquid chromatography
- high speed
- atomic force microscopy