CRISPR-Cas9 screens reveal common essential miRNAs in human cancer cell lines.

Daniel J Merk Linda Paul Foteini Tsiami Helen Hohenthanner Ghazal Mohseni Kouchesfahani Lara A Haeusser Bianca WalterAdam BrownNicole S Persky David E Root Ghazaleh Tabatabai

Published in: Genome medicine (2024)

Our study presents a miRNA-targeting CRISPR-Cas9 knockout library with high gene coverage and optimized on- and off-target activities. Taking advantage of the lentiG-miR library, we define a catalogue of miRNA fitness genes in human cancer cell lines, providing the foundation for further investigation of miRNAs in human cancer.

Keyphrases

crispr cas
endothelial cells
papillary thyroid
genome wide
genome editing
squamous cell
induced pluripotent stem cells
pluripotent stem cells
cell proliferation
physical activity
long non coding rna
healthcare
body composition
squamous cell carcinoma
transcription factor
young adults