Cationic lipid nanoparticle-mediated delivery of a Cas9/crRNA ribonucleoprotein complex for transgene-free editing of the citrus plant genome.

Published in: Plant cell reports (2024)

A lipofectamine-mediated transfection protocol for DNA-free genome editing of citrus protoplast cells using a Cas9/gRNA ribonucleoprotein (RNP) complex resulted in the production of transgene free genome edited citrus.

Keyphrases

crispr cas
genome editing
induced apoptosis
randomized controlled trial
genome wide
cell cycle arrest
gene expression
signaling pathway
oxidative stress
fatty acid
circulating tumor cells