Efficient scarless genome editing in human pluripotent stem cells.

Kazuya IkedaNobuko UchidaToshinobu NishimuraJoseph WhiteRenata M MartinHiromitsu NakauchiVittorio SebastianoKenneth I WeinbergMatthew H Porteus

Published in: Nature methods (2018)

Scarless genome editing in human pluripotent stem cells (hPSCs) represents a goal for both precise research applications and clinical translation of hPSC-derived therapies. Here we established a versatile and efficient method that combines CRISPR-Cas9-mediated homologous recombination with positive-negative selection of edited clones to generate scarless genetic changes in hPSCs.

Keyphrases

crispr cas
pluripotent stem cells
genome editing
dna damage
dna repair
endothelial cells
genome wide
gene expression
dna methylation