Herbicidins from Streptomyces sp. CB01388 Showing Anti- Cryptosporidium Activity.
Jian-Jun ChenMostafa E RatebMelissa S LoveZhengren XuDong YangXiangcheng ZhuYong HuangLi-Xing ZhaoYi JiangYanwen DuanCase W McNamaraBen ShenPublished in: Journal of natural products (2018)
A high-content imaging assay was used to screen the fraction collection of the Natural Product Library at The Scripps Research Institute for inhibitors of Cryptosporidium parvum. A chemical investigation of one strain, Streptomyces sp. CB01388, resulted in the isolation of six herbicidins (1-6), one of which is new (herbicidin L, 1). Five of the six herbicidins (1-3, 5, 6) showed moderate inhibitory activity against C. parvum, with 1 and 6 comparable to the FDA-approved drug nitazoxanide, and 2-6 showed no toxicity to the host HCT-8 cells and human HEK293T and HepG2 cells. These findings highlight the herbicidin scaffold for anti- Cryptosporidium drug development.
Keyphrases
- cell cycle arrest
- high throughput
- induced apoptosis
- endothelial cells
- oxidative stress
- cell death
- high intensity
- induced pluripotent stem cells
- signaling pathway
- pluripotent stem cells
- pi k akt
- emergency department
- drug induced
- photodynamic therapy
- cell proliferation
- single cell
- fluorescence imaging
- electronic health record
- oxide nanoparticles