Allium tuberosum -derived nanovesicles with anti-inflammatory properties prevent DSS-induced colitis and modify the gut microbiome.
Minkyoung KangMinji KangJuyeon LeeJiseon YooSujeong LeeSangnam OhPublished in: Food & function (2024)
Edible plant-derived nanovesicles (ePDNs) have shown potential as a non-pharmacological option for inflammatory bowel disease (IBD) by maintaining gut health and showing anti-inflammatory effects. However, the effects of Allium tuberosum -derived nanovesicles (ADNs) on colitis have not been studied to date. Here, we extracted exosome-like nanovesicles from Allium tuberosum and investigated whether they have an anti-inflammatory effect in RAW 264.7 cells and colitis mice. The results showed that ADNs reduced the elevated levels of inflammatory factors such as IL-1β, IL-6, TNF-α, and NF-κB pathway-related proteins as a consequence of lipopolysaccharide (LPS) stimulation in RAW 264.7 cells. Furthermore, our mouse experiments demonstrated that ADNs could ameliorate dextran sulfate sodium (DSS)-induced colitis symptoms ( e.g. , increased disease activity index score, intestinal permeability, and histological appearance). Additionally, ADNs counteracted DSS-induced colitis by downregulating the expression of serum amyloid A (SAA), IL-1β, IL-6, and TNF-α and increasing the expression of tight junction proteins (ZO-1 and occludin) and the anti-inflammatory cytokine IL-10. 16S rRNA gene sequencing showed that ADN intervention restored the gut microbial composition, which was similar to that of the DSS non-treated group, by decreasing the ratio of Firmicutes to Bacteroidetes and the relative abundance of Proteobacteria . Furthermore, ADNs induced acetic acid production along with an increase in the abundance of Lactobacillus . Overall, our findings suggest that ADN supplementation has a crucial role in maintaining gut health and is a novel preventive therapy for IBD.
Keyphrases
- anti inflammatory
- rheumatoid arthritis
- disease activity
- induced apoptosis
- public health
- healthcare
- poor prognosis
- systemic lupus erythematosus
- ulcerative colitis
- randomized controlled trial
- cell cycle arrest
- mental health
- oxidative stress
- inflammatory response
- signaling pathway
- type diabetes
- ankylosing spondylitis
- microbial community
- metabolic syndrome
- endothelial cells
- mass spectrometry
- toll like receptor
- endoplasmic reticulum stress
- pi k akt
- cell proliferation
- insulin resistance
- immune response
- single cell
- adipose tissue
- high fat diet induced
- atomic force microscopy
- high speed