Orally Administered Lactobacillus rhamnosus CY12 Alleviates DSS-Induced Colitis in Mice by Restoring the Intestinal Barrier and Inhibiting the TLR4-MyD88-NF-κB Pathway via Intestinal Microbiota Modulation.
Juanshan ZhengAnum Ali AhmadChen YangZeyi LiangWenxiang ShenJing LiuZuoting YanJianlin HanYayuan YangPengcheng DongXianyong LanGhasem Hosseini SalekdehXuezhi DingPublished in: Journal of agricultural and food chemistry (2024)
Oral ingestion of probiotics is a promising approach to relieving inflammatory disease through regulating the gut microbiota. A newly discovered strain, Lactobacillus rhamnosus CY12 (LCY12), obtained from cattle-yak milk, displayed numerous probiotic properties. These included enhanced viability in low pH and bile environments, adhesion capabilities, and potent antimicrobial effects. The research aimed to explore the beneficial impacts of the novel LCY12 strain on colitis in mice induced by dextran sulfate sodium (DSS) and to elucidate the underlying molecular mechanisms. The results of the study showed that administration of LCY12 effectively helped to reduce the negative effects of DSS-induced body weight loss, disease activity index score, colon length shortening, loss of goblet cells, and overall histopathological scores in the intestines. Simultaneously, LCY12 administration significantly alleviated intestinal inflammation and safeguarded intestinal barrier integrity by enhancing IL-10 levels, while dampening IL-6, IL-1β, and TNF-α production. Additionally, LCY12 boosted the presence of tight junction proteins. Furthermore, LCY12 hindered the TLR4/MyD88/NF-κB signaling pathway by downregulating TLR4 and MyD88 expression, inactivating phosphorylated IκBα, and preventing translocation of NF-κB p65 from the cytoplasm to the nucleus. The LCY12 also increased specific intestinal microbial communities and short-chain fatty acid (SCFA) production. Altogether, LCY12 oral administration alleviated colitis induced with DSS in mice by improving intestinal barrier function and regulating inflammatory cytokines, SCFA production, and intestinal microbiota.
Keyphrases
- signaling pathway
- toll like receptor
- induced apoptosis
- nuclear factor
- oxidative stress
- disease activity
- inflammatory response
- rheumatoid arthritis
- pi k akt
- lps induced
- weight loss
- immune response
- high fat diet induced
- diabetic rats
- systemic lupus erythematosus
- high glucose
- fatty acid
- poor prognosis
- type diabetes
- blood brain barrier
- ankylosing spondylitis
- metabolic syndrome
- rheumatoid arthritis patients
- drug induced
- adipose tissue
- endothelial cells
- endoplasmic reticulum stress
- mouse model
- binding protein
- long non coding rna
- gastric bypass
- weight gain
- glycemic control