Homologous Recombination-Enhancing Factors Identified by Comparative Transcriptomic Analyses of Pluripotent Stem Cell of Human and Common Marmoset.
Sho YoshimatsuMayutaka NakajimaEmi QianTsukasa SanosakaTsukika SatoHideyuki OkanoPublished in: Cells (2022)
A previous study assessing the efficiency of the genome editing technology CRISPR-Cas9 for knock-in gene targeting in common marmoset (marmoset; Callithrix jacchus) embryonic stem cells (ESCs) unexpectedly identified innately enhanced homologous recombination activity in marmoset ESCs. Here, we compared gene expression in marmoset and human pluripotent stem cells using transcriptomic and quantitative PCR analyses and found that five HR-related genes (BRCA1, BRCA2, RAD51C, RAD51D, and RAD51) were upregulated in marmoset cells. A total of four of these upregulated genes enhanced HR efficiency with CRISPR-Cas9 in human pluripotent stem cells. Thus, the present study provides a novel insight into species-specific mechanisms for the choice of DNA repair pathways.
Keyphrases
- dna repair
- pluripotent stem cells
- crispr cas
- genome editing
- dna damage
- endothelial cells
- gene expression
- dna damage response
- stem cells
- genome wide
- dna methylation
- single cell
- induced pluripotent stem cells
- drug delivery
- high resolution
- induced apoptosis
- cell death
- rna seq
- copy number
- decision making
- transcription factor
- breast cancer risk
- cancer therapy