Anti-adipogenic Effects of the Probiotic Lactiplantibacillus plantarum KU15117 on 3T3-L1 Adipocytes.
Kyoung Jun HanNa-Kyoung LeeHyung-Seok YuHoon ParkHyun-Dong PaikPublished in: Probiotics and antimicrobial proteins (2021)
In this study, we investigated the probiotic properties and anti-obesity effects of bacterial strains isolated from homemade kimchi. Lactiplantibacillus plantarum KU15117 was isolated using lactobacilli selective medium. L. plantarum KU15117 did not produce β-glucuronidase and showed high tolerance to artificial gastric juice and bile salt, acceptable resistance to antibiotics, and high adhesion ability to HT-29 cells. The anti-adipogenic activity of L. plantarum KU15117 at 109 CFU/well was confirmed by the reduction of oil red O staining and intracellular triglyceride level. Additionally, the expression levels of fatty acid synthase, CCAAT/enhance-binding protein-α, and peroxisome proliferator-activated receptor-γ, which are associated with the early stage of adipocyte differentiation, were significantly lower in the probiotic-treated group than in the control group. These results suggest that L. plantarum KU15117 has probiotic properties and anti-obesity effects and could be used as a prophylactic probiotics.
Keyphrases
- fatty acid
- insulin resistance
- binding protein
- early stage
- metabolic syndrome
- adipose tissue
- type diabetes
- weight loss
- bacillus subtilis
- poor prognosis
- escherichia coli
- induced apoptosis
- weight gain
- squamous cell carcinoma
- body mass index
- cystic fibrosis
- pseudomonas aeruginosa
- cell proliferation
- biofilm formation
- atomic force microscopy