Immortalization of human primary prostate epithelial cells via CRISPR inactivation of the CDKN2A locus and expression of telomerase.
Ziran ZhaoHolly FowleHenkel ValentineZemin LiuYinfei TanJianming PeiSimone Ann Marie BadalJoseph R TestaXavier GrañaPublished in: Prostate cancer and prostatic diseases (2020)
Use of this approach resulted in the immortalization of independent clones of PrEC that retained normal characteristics, were stable, and non-transformed. Thus, this approach could be used for the immortalization of normal primary prostate cells. This technique could also be useful for establishing cell lines from prostate tumor tissues of different tumor grades and/or from patients of diverse ethnicities to generate cell line models that facilitate the study of the molecular basis of disease disparity.
Keyphrases
- prostate cancer
- benign prostatic hyperplasia
- end stage renal disease
- endothelial cells
- induced apoptosis
- ejection fraction
- poor prognosis
- newly diagnosed
- chronic kidney disease
- gene expression
- crispr cas
- prognostic factors
- peritoneal dialysis
- genome wide
- genome editing
- cell cycle arrest
- oxidative stress
- cell death
- pluripotent stem cells