Burkhard Ludewig

Publication Activity (10 Years)

Years Active: 2017-2024
Publications (10 Years): 53

Top Topics

Immune Response

Top Venues

Nature communications

Journal of immunology (Baltimore, Md. : 1950)

Nature immunology

Science immunology

This page only lists publications with an associated author ORCID identifier.

Publications

Irma Telarovic, Carmen S M Yong, Lisa Kurz, Irene Vetrugno, Sabrina Reichl, Alba Sanchez Fernandez, Hung-Wei Cheng, Rona Winkler, Matthias Guckenberger, Anja Kipar, Burkhard Ludewig, Martin Pruschy
Delayed tumor-draining lymph node irradiation preserves the efficacy of combined radiotherapy and immune checkpoint blockade in models of metastatic disease. Nature communications 15 (1) (2024)
Christian Perez-Shibayama, Cristina Gil-Cruz, Nadine Cadosch, Mechthild Lütge, Hung-Wei Cheng, Angelina De Martin, Kira Frischmann, Anna Joachimbauer, Lucas Onder, Iliana Papadopoulou, Chrysa Papadopoulou, Sandra Ring, Philippe Krebs, Vivian P Vu, Matthias P Nägele, Valentina A Rossi, Danaë Parianos, Valentin W Zsilavecz, Leslie T Cooper, Andreas Flammer, Frank Ruschitzka, Peter P Rainer, Dörthe Schmidt, Burkhard Ludewig
Bone morphogenic protein-4 availability in the cardiac microenvironment controls inflammation and fibrosis in autoimmune myocarditis. Nature cardiovascular research 3 (3) (2024)
Nadine Cadosch, Cristina Gil-Cruz, Christian Perez-Shibayama, Burkhard Ludewig
Cardiac Fibroblastic Niches in Homeostasis and Inflammation. Circulation research 134 (12) (2024)
Christian Perez-Shibayama, Cristina Gil-Cruz, Nadine Cadosch, Mechthild Lütge, Hung-Wei Cheng, Angelina De Martin, Kira Frischmann, Anna Joachimbauer, Lucas Onder, Iliana Papadopoulou, Chrysa Papadopoulou, Sandra Ring, Philippe Krebs, Vivian P Vu, Matthias P Nägele, Valentina A Rossi, Danaë Parianos, Valentin W Zsilavecz, Leslie T Cooper, Andreas Flammer, Frank Ruschitzka, Peter P Rainer, Dörthe Schmidt, Burkhard Ludewig
Bone morphogenic protein-4 availability in the cardiac microenvironment controls inflammation and fibrosis in autoimmune myocarditis. Nature cardiovascular research 3 (3) (2024)
Nadine Cadosch, Cristina Gil-Cruz, Christian Perez-Shibayama, Burkhard Ludewig
Cardiac Fibroblastic Niches in Homeostasis and Inflammation. Circulation research 134 (12) (2024)
Christian Perez-Shibayama, Cristina Gil-Cruz, Nadine Cadosch, Mechthild Lütge, Hung-Wei Cheng, Angelina De Martin, Kira Frischmann, Anna Joachimbauer, Lucas Onder, Iliana Papadopoulou, Chrysa Papadopoulou, Sandra Ring, Philippe Krebs, Vivian P Vu, Matthias P Nägele, Valentina A Rossi, Danaë Parianos, Valentin W Zsilavecz, Leslie T Cooper, Andreas Flammer, Frank Ruschitzka, Peter P Rainer, Dörthe Schmidt, Burkhard Ludewig
Bone morphogenic protein-4 availability in the cardiac microenvironment controls inflammation and fibrosis in autoimmune myocarditis. Nature cardiovascular research 3 (3) (2024)
Nadine Cadosch, Cristina Gil-Cruz, Christian Perez-Shibayama, Burkhard Ludewig
Cardiac Fibroblastic Niches in Homeostasis and Inflammation. Circulation research 134 (12) (2024)
Irma Telarovic, Carmen S M Yong, Lisa Kurz, Irene Vetrugno, Sabrina Reichl, Alba Sanchez Fernandez, Hung-Wei Cheng, Rona Winkler, Matthias Guckenberger, Anja Kipar, Burkhard Ludewig, Martin Pruschy
Delayed tumor-draining lymph node irradiation preserves the efficacy of combined radiotherapy and immune checkpoint blockade in models of metastatic disease. Nature communications 15 (1) (2024)
Irma Telarovic, Carmen S M Yong, Lisa Kurz, Irene Vetrugno, Sabrina Reichl, Alba Sanchez Fernandez, Hung-Wei Cheng, Rona Winkler, Matthias Guckenberger, Anja Kipar, Burkhard Ludewig, Martin Pruschy
Delayed tumor-draining lymph node irradiation preserves the efficacy of combined radiotherapy and immune checkpoint blockade in models of metastatic disease. Nature communications 15 (1) (2024)
Irma Telarovic, Carmen S M Yong, Lisa Kurz, Irene Vetrugno, Sabrina Reichl, Alba Sanchez Fernandez, Hung-Wei Cheng, Rona Winkler, Matthias Guckenberger, Anja Kipar, Burkhard Ludewig, Martin Pruschy
Delayed tumor-draining lymph node irradiation preserves the efficacy of combined radiotherapy and immune checkpoint blockade in models of metastatic disease. Nature communications 15 (1) (2024)
Mechthild Lütge, Angelina De Martin, Cristina Gil-Cruz, Christian Perez-Shibayama, Yves Stanossek, Lucas Onder, Hung-Wei Cheng, Lisa Kurz, Nadine Cadosch, Charlotte Soneson, Mark D Robinson, Sandro J Stoeckli, Burkhard Ludewig, Natalia Barbara Pikor
Conserved stromal-immune cell circuits secure B cell homeostasis and function. Nature immunology (2023)
Sarah Grabherr, Alexandra Waltenspühl, Lorina Büchler, Mechthild Lütge, Hung-Wei Cheng, Sonja Caviezel-Firner, Burkhard Ludewig, Philippe Krebs, Natalia Barbara Pikor
An Innate Checkpoint Determines Immune Dysregulation and Immunopathology during Pulmonary Murine Coronavirus Infection. Journal of immunology (Baltimore, Md. : 1950) 210 (6) (2023)
Angelina De Martin, Yves Stanossek, Natalia Barbara Pikor, Burkhard Ludewig
Protective fibroblastic niches in secondary lymphoid organs. The Journal of experimental medicine 221 (1) (2023)
Victor Tkachev, Ashley Vanderbeck, Eric Perkey, Scott N Furlan, Connor McGuckin, Daniela Gómez Atria, Ulrike Gerdemann, Xianliang Rui, Jennifer Lane, Daniel J Hunt, Hengqi Betty Zheng, Lucrezia Colonna, Michelle Hoffman, Alison Yu, Riley Outen, Samantha Kelly, Anneka Allman, Ute Koch, Freddy Radtke, Burkhard Ludewig, Brandon J Burbach, Yoji Shimizu, Angela Panoskaltsis-Mortari, Guoying Chen, Stephen M Carpenter, Olivier Harari, Frank Kuhnert, Gavin Thurston, Bruce R Blazar, Leslie S Kean, Ivan P Maillard
Notch signaling drives intestinal graft-versus-host disease in mice and nonhuman primates. Science translational medicine 15 (702) (2023)
Sarah Grabherr, Alexandra Waltenspühl, Lorina Büchler, Mechthild Lütge, Hung-Wei Cheng, Sonja Caviezel-Firner, Burkhard Ludewig, Philippe Krebs, Natalia Barbara Pikor
An Innate Checkpoint Determines Immune Dysregulation and Immunopathology during Pulmonary Murine Coronavirus Infection. Journal of immunology (Baltimore, Md. : 1950) 210 (6) (2023)
Angelina De Martin, Yves Stanossek, Mechthild Lütge, Nadine Cadosch, Lucas Onder, Hung-Wei Cheng, Joshua D Brandstadter, Ivan P Maillard, Sandro J Stoeckli, Natalia Barbara Pikor, Burkhard Ludewig
PI16 + reticular cells in human palatine tonsils govern T cell activity in distinct subepithelial niches. Nature immunology (2023)
Victor Tkachev, Ashley Vanderbeck, Eric Perkey, Scott N Furlan, Connor McGuckin, Daniela Gómez Atria, Ulrike Gerdemann, Xianliang Rui, Jennifer Lane, Daniel J Hunt, Hengqi Betty Zheng, Lucrezia Colonna, Michelle Hoffman, Alison Yu, Riley Outen, Samantha Kelly, Anneka Allman, Ute Koch, Freddy Radtke, Burkhard Ludewig, Brandon J Burbach, Yoji Shimizu, Angela Panoskaltsis-Mortari, Guoying Chen, Stephen M Carpenter, Olivier Harari, Frank Kuhnert, Gavin Thurston, Bruce R Blazar, Leslie S Kean, Ivan P Maillard
Notch signaling drives intestinal graft-versus-host disease in mice and nonhuman primates. Science translational medicine 15 (702) (2023)
Susanne Sattler, Gustavo Campos Ramos, Burkhard Ludewig, Peter P Rainer
Cardioimmunology: the new frontier! European heart journal (2023)
Sarah Grabherr, Alexandra Waltenspühl, Lorina Büchler, Mechthild Lütge, Hung-Wei Cheng, Sonja Caviezel-Firner, Burkhard Ludewig, Philippe Krebs, Natalia Barbara Pikor
An Innate Checkpoint Determines Immune Dysregulation and Immunopathology during Pulmonary Murine Coronavirus Infection. Journal of immunology (Baltimore, Md. : 1950) (2023)
Sarah Grabherr, Alexandra Waltenspühl, Lorina Büchler, Mechthild Lütge, Hung-Wei Cheng, Sonja Caviezel-Firner, Burkhard Ludewig, Philippe Krebs, Natalia Barbara Pikor
An Innate Checkpoint Determines Immune Dysregulation and Immunopathology during Pulmonary Murine Coronavirus Infection. Journal of immunology (Baltimore, Md. : 1950) 210 (6) (2023)
Joshua D Brandstadter, Angelina De Martin, Mechthild Lütge, Antonio Ferreira, Brian T Gaudette, Yves Stanossek, Shumei Wang, Michael V Gonzalez, Edward Camiolo, Gerald Wertheim, Bridget Austin, David Allman, Megan S Lim, David C Fajgenbaum, Jon C Aster, Burkhard Ludewig, Ivan P Maillard
A novel cryopreservation and biobanking strategy to study lymphoid tissue stromal cells in human disease. bioRxiv : the preprint server for biology (2023)
Ana Martínez-Riaño, Shenshen Wang, Stefan Boeing, Sophie Minoughan, Antonio Casal, Katelyn M Spillane, Burkhard Ludewig, Pavel Tolar
Long-term retention of antigens in germinal centers is controlled by the spatial organization of the follicular dendritic cell network. Nature immunology (2023)
Ana Martínez-Riaño, Shenshen Wang, Stefan Boeing, Sophie Minoughan, Antonio Casal, Katelyn M Spillane, Burkhard Ludewig, Pavel Tolar
Author Correction: Long-term retention of antigens in germinal centers is controlled by the spatial organization of the follicular dendritic cell network. Nature immunology (2023)
Victor Tkachev, Ashley Vanderbeck, Eric Perkey, Scott N Furlan, Connor McGuckin, Daniela Gómez Atria, Ulrike Gerdemann, Xianliang Rui, Jennifer Lane, Daniel J Hunt, Hengqi Betty Zheng, Lucrezia Colonna, Michelle Hoffman, Alison Yu, Riley Outen, Samantha Kelly, Anneka Allman, Ute Koch, Freddy Radtke, Burkhard Ludewig, Brandon J Burbach, Yoji Shimizu, Angela Panoskaltsis-Mortari, Guoying Chen, Stephen M Carpenter, Olivier Harari, Frank Kuhnert, Gavin Thurston, Bruce R Blazar, Leslie S Kean, Ivan P Maillard
Notch signaling drives intestinal graft-versus-host disease in mice and nonhuman primates. Science translational medicine 15 (702) (2023)
Sarah Grabherr, Alexandra Waltenspühl, Lorina Büchler, Mechthild Lütge, Hung-Wei Cheng, Sonja Caviezel-Firner, Burkhard Ludewig, Philippe Krebs, Natalia Barbara Pikor
An Innate Checkpoint Determines Immune Dysregulation and Immunopathology during Pulmonary Murine Coronavirus Infection. Journal of immunology (Baltimore, Md. : 1950) 210 (6) (2023)
Yannick O Alexandre, Dominik Schienstock, Hyun Jae Lee, Luke C Gandolfo, Cameron G Williams, Sapna Devi, Bhupinder Pal, Joanna R Groom, Wang H J Cao, Susan N Christo, Claire L Gordon, Graham Starkey, Rohit D'Costa, Laura K Mackay, Ashraful Haque, Burkhard Ludewig, Gabrielle T Belz, Scott N Mueller
A diverse fibroblastic stromal cell landscape in the spleen directs tissue homeostasis and immunity. Science immunology 7 (67) (2022)
Hung-Wei Cheng, Urs M Mörbe, Mechthild Lütge, Céline Engetschwiler, Lucas Onder, Mario Novkovic, Cristina Gil-Cruz, Christian Perez-Shibayama, Thomas Hehlgans, Elke Scandella, Burkhard Ludewig
Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function. Nature communications 13 (1) (2022)
Frank P Assen, Jun Abe, Miroslav Hons, Robert Hauschild, Shayan Shamipour, Walter A Kaufmann, Tommaso Costanzo, Gabriel Krens, Markus Brown, Burkhard Ludewig, Simon Hippenmeyer, Carl-Philipp Heisenberg, Wolfgang Weninger, Edouard Hannezo, Sanjiv A Luther, Jens V Stein, Michael Sixt
Multitier mechanics control stromal adaptations in the swelling lymph node. Nature immunology 23 (8) (2022)
Christian Perez-Shibayama, Ulrika Islander, Mechthild Lütge, Hung-Wei Cheng, Lucas Onder, Sandra S Ring, Angelina De Martin, Mario Novkovic, Julia Colston, Cristina Gil-Cruz, Burkhard Ludewig
Type I interferon signaling in fibroblastic reticular cells prevents exhaustive activation of antiviral CD8+ T cells. Science immunology 5 (51) (2022)
Sarah Davidson, Mark Coles, Tom Thomas, George Kollias, Burkhard Ludewig, Shannon J Turley, Michael Brenner, Christopher Dominic Buckley
Fibroblasts as immune regulators in infection, inflammation and cancer. Nature reviews. Immunology 21 (11) (2021)
Angelina De Martin, Mechthild Lütge, Yves Stanossek, Céline Engetschwiler, Jovana Cupovic, Kirsty Brown, Izadora Demmer, Martina A Broglie, Markus B Geuking, Wolfram Jochum, Kathy D McCoy, Sandro J Stoeckli, Burkhard Ludewig
Distinct microbial communities colonize tonsillar squamous cell carcinoma. Oncoimmunology 10 (1) (2021)
Sandra S Ring, Jovana Cupovic, Lucas Onder, Mechthild Lütge, Christian Perez-Shibayama, Cristina Gil-Cruz, Elke Scandella, Angelina De Martin, Urs M Mörbe, Fabienne Hartmann, Robert Wenger, Matthias K F Spiegl, Andrej Besse, Weldy V Bonilla, Felix Stemeseder, Sarah Schmidt, Klaus K Orlinger, Philippe Krebs, Burkhard Ludewig, Lukas Flatz
Viral vector-mediated reprogramming of the fibroblastic tumor stroma sustains curative melanoma treatment. Nature communications 12 (1) (2021)
Alejandro Prados, Lucas Onder, Hung-Wei Cheng, Urs M Mörbe, Mechthild Lütge, Cristina Gil-Cruz, Christian Perez-Shibayama, Vasiliki Koliaraki, Burkhard Ludewig, George Kollias
Fibroblastic reticular cell lineage convergence in Peyer's patches governs intestinal immunity. Nature immunology 22 (4) (2021)
Mechthild Lütge, Natalia B Pikor, Burkhard Ludewig
Differentiation and activation of fibroblastic reticular cells. Immunological reviews 302 (1) (2021)
Natalia Barbara Pikor, Hung-Wei Cheng, Lucas Onder, Burkhard Ludewig
Development and Immunological Function of Lymph Node Stromal Cells. Journal of immunology (Baltimore, Md. : 1950) 206 (2) (2021)
Lucas Onder, Hung-Wei Cheng, Burkhard Ludewig
Visualization and functional characterization of lymphoid organ fibroblasts. Immunological reviews (2021)
Sarah Grabherr, Burkhard Ludewig, Natalia Barbara Pikor
Insights into coronavirus immunity taught by the murine coronavirus. European journal of immunology 51 (5) (2021)
Fiamma Berner, Rebekka Niederer, Jolien J Luimstra, Oltin Tiberiu Pop, Ann-Kristin Jochum, Mette-Triin Purde, Omar Hasan Ali, David Bomze, Jens Bauer, Lena Katharina Freudenmann, Ana Marcu, Eva-Maria Wolfschmitt, Sebastian Haen, Thorben Gross, Marissa Lisa Dubbelaar, Marie-Therese Abdou, Petra Baumgaertner, Christina Appenzeller, Caroline Cicin-Sain, Tobias Lenz, Daniel E Speiser, Burkhard Ludewig, Christoph Driessen, Markus Jörger, Martin Früh, Wolfram Jochum, Antonio Cozzio, Hans-Georg Rammensee, Juliane Walz, Jacques Neefjes, Lukas Flatz
Keratinocyte differentiation antigen-specific T cells in immune checkpoint inhibitor-treated NSCLC patients are associated with improved survival. Oncoimmunology 10 (1) (2021)
Jovana Cupovic, Sandra S Ring, Lucas Onder, Julia M Colston, Mechthild Lütge, Hung-Wei Cheng, Angelina De Martin, Nicholas M Provine, Lukas Flatz, Annette Oxenius, Elke Scandella, Philippe Krebs, Daniel S Engeler, Paul Klenerman, Burkhard Ludewig
Adenovirus vector vaccination reprograms pulmonary fibroblastic niches to support protective inflating memory CD8+ T cells. Nature immunology 22 (8) (2021)
Natalia B Pikor, Urs M Mörbe, Mechthild Lütge, Cristina Gil-Cruz, Christian Perez-Shibayama, Mario Novkovic, Hung-Wei Cheng, Cesar Nombela-Arrieta, Takashi Nagasawa, Michelle A Linterman, Lucas Onder, Burkhard Ludewig
Remodeling of light and dark zone follicular dendritic cells governs germinal center responses. Nature immunology 21 (6) (2020)
Cristina Gil-Cruz, Christian Perez-Shibayama, Angelina De Martin, Francesca Ronchi, Katrien van der Borght, Rebekka Niederer, Lucas Onder, Mechthild Lütge, Mario Novkovic, Veronika Nindl, Gustavo Campos Ramos, Markus Arnoldini, Emma M C Slack, Valérie Boivin-Jahns, Roland Jahns, Madeleine Wyss, Catherine Mooser, Bart N Lambrecht, Micha T Maeder, Hans Rickli, Lukas Flatz, Urs Eriksson, Markus B Geuking, Kathy D McCoy, Burkhard Ludewig
Microbiota-derived peptide mimics drive lethal inflammatory cardiomyopathy. Science (New York, N.Y.) 366 (6467) (2020)
Jason Cosgrove, Mario Novkovic, Stefan Albrecht, Natalia B Pikor, Zhaoukun Zhou, Lucas Onder, Urs Mörbe, Jovana Cupovic, Helen Miller, Kieran Alden, Anne Thuery, Peter O'Toole, Rita Pinter, Simon Jarrett, Emily Taylor, Daniel Venetz, Manfred Heller, Mariagrazia Uguccioni, Daniel F Legler, Charles J Lacey, Andrew Coatesworth, Wojciech G Polak, Tom Cupedo, Bénedicte Manoury, Marcus Thelen, Jens V Stein, Marlene Wolf, Mark C Leake, Jon Timmis, Burkhard Ludewig, Mark C Coles
B cell zone reticular cell microenvironments shape CXCL13 gradient formation. Nature communications 11 (1) (2020)
Sung Yong Choi, Hosung Bae, Sun-Hye Jeong, Intae Park, Hyunsoo Cho, Seon Pyo Hong, Da-Hye Lee, Choong-Kun Lee, Jin-Sung Park, Sang Heon Suh, Jeongwoon Choi, Myung Jin Yang, Jeon Yeob Jang, Lucas Onder, Jeong Hwan Moon, Han-Sin Jeong, Ralf H Adams, Jin-Man Kim, Burkhard Ludewig, Joo-Hye Song, Dae-Sik Lim, Gou Young Koh
YAP/TAZ direct commitment and maturation of lymph node fibroblastic reticular cells. Nature communications 11 (1) (2020)
Conglei Li, Evelyn Lam, Christian Perez-Shibayama, Lesley A Ward, Jianbo Zhang, Dennis S W Lee, Albert Nguyen, Musaddeque Ahmed, Emma Brownlie, Kirill V Korneev, Olga L Rojas, Tian Sun, William Wiley Navarre, Housheng Hansen He, Shan Liao, Alberto Martin, Burkhard Ludewig, Jennifer L Gommerman
Early-life programming of mesenteric lymph node stromal cell identity by the lymphotoxin pathway regulates adult mucosal immunity. Science immunology 4 (42) (2020)
Christian Perez-Shibayama, Cristina Gil-Cruz, Burkhard Ludewig
Fibroblastic reticular cells at the nexus of innate and adaptive immune responses. Immunological reviews 289 (1) (2020)
Dragos C Dasoveanu, Hyeung Ju Park, Catherine L Ly, William D Shipman, Susan Chyou, Varsha Kumar, David Mathew Tarlinton, Burkhard Ludewig, Babak J Mehrara, Theresa T Lu
Lymph node stromal CCL2 limits antibody responses. Science immunology 5 (45) (2020)
Eric Perkey, Dave Maurice De Sousa, Léolène J Carrington, Jooho Chung, Alexander Dils, David Granadier, Ute Koch, Freddy Radtke, Burkhard Ludewig, Bruce R Blazar, Christian W Siebel, Todd V Brennan, Jeffrey C Nolz, Nathalie Labrecque, Ivan P Maillard
GCNT1-Mediated O-Glycosylation of the Sialomucin CD43 Is a Sensitive Indicator of Notch Signaling in Activated T Cells. Journal of immunology (Baltimore, Md. : 1950) 204 (6) (2020)
Hung-Wei Cheng, Lucas Onder, Mario Novkovic, Charlotte Soneson, Mechthild Lütge, Natalia Pikor, Elke Scandella, Mark D Robinson, Jun-Ichi Miyazaki, Anne Tersteegen, Ursula Sorg, Klaus Pfeffer, Thomas Rülicke, Thomas Hehlgans, Burkhard Ludewig
Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp. Nature communications 10 (1) (2019)
Alice E Denton, Silvia Innocentin, Edward J Carr, Barry M Bradford, Fanny Lafouresse, Neil A Mabbott, Urs M Mörbe, Burkhard Ludewig, Joanna R Groom, Kim L Good-Jacobson, Michelle A Linterman
Type I interferon induces CXCL13 to support ectopic germinal center formation. The Journal of experimental medicine 216 (3) (2019)
Christian Perez-Shibayama, Cristina Gil-Cruz, Hung-Wei Cheng, Lucas Onder, Andrea Printz, Urs M Mörbe, Mario Novkovic, Conglei Li, Constantino Lopez-Macias, Matthew B Buechler, Shannon J Turley, Matthias Mack, Charlotte Soneson, Mark D Robinson, Elke Scandella, Jennifer L Gommerman, Burkhard Ludewig
Fibroblastic reticular cells initiate immune responses in visceral adipose tissues and secure peritoneal immunity. Science immunology 3 (26) (2019)
Burkhard Ludewig
Legends of allergy/immunology: Rolf Zinkernagel and the co-discovery of MHC restriction together with Peter Doherty. Allergy 74 (7) (2019)
Dana Bogdanova, Arata Takeuchi, Madoka Ozawa, Yasuhiro Kanda, M Azizur Rahman, Burkhard Ludewig, Tatsuo Kinashi, Tomoya Katakai
Essential Role of Canonical NF-κB Activity in the Development of Stromal Cell Subsets in Secondary Lymphoid Organs. Journal of immunology (Baltimore, Md. : 1950) 201 (12) (2018)
Juan Manuel Carreño, Christian Perez-Shibayama, Cristina Gil-Cruz, Constantino Lopez-Macias, Pietro Vernazza, Burkhard Ludewig, Werner C Albrich
Evolution of Salmonella Typhi outer membrane protein-specific T and B cell responses in humans following oral Ty21a vaccination: A randomized clinical trial. PloS one 12 (6) (2017)