Nanoencapsulation Motivates the High Inhibitive Ability of Fucoxanthin on H 2 O 2 -Induced Human Hepatocyte Cell Line (L02) Apoptosis via Regulating Lipid Metabolism Homeostasis.

This study reports an encapsulation system for fucoxanthin (FX) through simple affinity binding with gelatin (GE) and then coating with chitosan oligosaccharides (COS). The effects of FX before and after encapsulation on the human hepatocyte cell line (L02) were investigated. FX-GE and FX-GE-COS nanocomplexes exhibited a spherical shape with diameters of 209 ± 6 to 210 ± 8 nm. FX-GE-COS nanocomplexes were found to perform the best with the highest encapsulation efficiency (EE, 83.88 ± 4.39%), improved FX stability, and enhanced cellular uptake on the nanoscale. The cytotoxicity and cell mitochondrial damage of H 2 O 2 exposure to L02 cells decreased with the increase of free-FX and FX-GE-COS nanocomplexes. FX-GE-COS nanocomplexes' intervention decreased the intracellular ROS and inhibited the apoptosis of L02 cells that was induced by H 2 O 2 exposure in a concentration-dependent manner. Lipidomic analysis revealed that FX-GE-COS nanocomplexes could regulate the lipid metabolism disturbed by H 2 O 2 and protected the mitochondrial function of L02 cells. These results suggested that nanoencapsulation enhanced the antioxidant activity of FX to L02 cells, and the constructed FX-GE-COS nanocomplexes have the potential to be an antioxidant nutritional dietary supplement.