The Impact of Controlled Ovarian Stimulation Hormones on the Metabolic State and Endocannabinoid System of Human Cumulus Cells.
Valentina NotarstefanoGiorgia GioacchiniElisabetta GiorginiNina MontikAndrea CiavattiniAnna Rita PolidoriFulvia Antonia CandelaLisa VaccariMaurizio CignittiOliana CarnevaliPublished in: International journal of molecular sciences (2020)
Different Follicle Stimulating Hormone (FSH) formulation and Luteinizing Hormone (LH) are used in Assisted Reproductive Technology (ART) to induce follicles development and oocytes maturation, but it is still under debate which protocol is to be preferred. In the present study, the different effects on cumulus cells (CCs) of three controlled ovarian stimulation (COS) protocols, based on urinary FSH, recombinant FSH, or human Menopausal Gonadotropin (hMG) administration, were assessed. CCs were obtained from 42 normal-responders women undergoing COS, randomly divided into three groups according to the used gonadotropin formulation. Differences were found in the expression of genes belonging to the endocannabinoid system (the receptors CNR1, CNR2 and TRPV1, and the enzymes involved in the metabolisms of anandamide, NAPE-PLD and FAAH, and 2-acylglycerol, DAGL and MAGL); consistently, changes in lipid (PPARα, and FASN) and carbohydrate (GLUT1 and GLUT9) metabolisms, in CCs' macromolecules composition (highlighted by Fourier Transform Infrared Microspectroscopy, FTIRM), and in the number of retrieved oocytes were found. For the first time, statistically significant evidence on the differences related to each COS protocol on the endocannabinoid system, metabolism and macromolecular composition of CCs was found, representing a proof of concept to be further confirmed in a larger cohort of patients.
Keyphrases
- induced apoptosis
- endothelial cells
- cell cycle arrest
- end stage renal disease
- randomized controlled trial
- drug delivery
- induced pluripotent stem cells
- poor prognosis
- ejection fraction
- chronic kidney disease
- newly diagnosed
- endoplasmic reticulum stress
- cell death
- prognostic factors
- pluripotent stem cells
- genome wide
- polycystic ovary syndrome
- fatty acid
- spinal cord
- patient reported outcomes
- peritoneal dialysis
- pregnant women
- long non coding rna
- adipose tissue
- dna methylation
- pi k akt