Metabolic Fate of 13C-Labeled Polydextrose and Impact on the Gut Microbiome: A Triple-Phase Study in a Colon Simulator.
Santosh LamichhaneChristian C YdeHenrik Max JensenWesley MorovicAshley A HibberdArthur C OuwehandMarkku T SaarinenSofia D ForsstenLars WiebeJørn MarcussenKresten BertelsenSebastian MeierJette F YoungHanne Christine BertramPublished in: Journal of proteome research (2018)
The present study introduces a novel triple-phase (liquids, solids, and gases) approach, which employed uniformly labeled [U-13C] polydextrose (PDX) for the selective profiling of metabolites generated from dietary fiber fermentation in an in vitro colon simulator using human fecal inocula. Employing 13C NMR spectroscopy, [U-13C] PDX metabolism was observed from colonic digest samples. The major 13C-labeled metabolites generated were acetate, butyrate, propionate, and valerate. In addition to these short-chain fatty acids (SCFAs), 13C-labeled lactate, formate, succinate, and ethanol were detected in the colon simulator samples. Metabolite formation and PDX substrate degradation were examined comprehensively over time (24 and 48 h). Correlation analysis between 13C NMR spectra and gas production confirmed the anaerobic fermentation of PDX to SCFAs. In addition, 16S rRNA gene analysis showed that the level of Erysipelotrichaceae was influenced by PDX supplementation and Erysipelotrichaceae level was statistically correlated with SCFA formation. Overall, our study demonstrates a novel approach to link substrate fermentation and microbial function directly in a simulated colonic environment.
Keyphrases
- pet imaging
- microbial community
- fatty acid
- ms ms
- magnetic resonance
- saccharomyces cerevisiae
- randomized controlled trial
- virtual reality
- lactic acid
- high resolution
- copy number
- open label
- amino acid
- mass spectrometry
- transcription factor
- dna methylation
- study protocol
- positron emission tomography
- double blind
- genome wide identification