Mass spectrometry reveals potential of β-lactams as SARS-CoV-2 Mpro inhibitors.
Tika R MallaAnthony TumberTobias JohnLennart BrewitzClaire Strain-DamerellC David OwenPetra LukacikH T Henry ChanPratheesh MaheswaranEidarus SalahFernanda DuarteHaitao YangZihe RaoMartin Austin WalshChristopher J SchofieldPublished in: Chemical communications (Cambridge, England) (2021)
The main viral protease (Mpro) of SARS-CoV-2 is a nucleophilic cysteine hydrolase and a current target for anti-viral chemotherapy. We describe a high-throughput solid phase extraction coupled to mass spectrometry Mpro assay. The results reveal some β-lactams, including penicillin esters, are active site reacting Mpro inhibitors, thus highlighting the potential of acylating agents for Mpro inhibition.
Keyphrases
- sars cov
- mass spectrometry
- liquid chromatography
- high throughput
- high performance liquid chromatography
- solid phase extraction
- gas chromatography
- respiratory syndrome coronavirus
- tandem mass spectrometry
- liquid chromatography tandem mass spectrometry
- single cell
- molecularly imprinted
- high resolution mass spectrometry
- capillary electrophoresis
- high resolution
- ultra high performance liquid chromatography
- genome wide
- squamous cell carcinoma
- locally advanced
- coronavirus disease
- radiation therapy
- living cells
- fluorescent probe