Intervention Effects of Deer-Tendon Collagen Hydrolysates on Osteoporosis In Vitro and In Vivo.
Chaoting WenDan WangZhiyi ZhangGuoyan LiuLi LiangXiaofang LiuJixian ZhangYoudong LiXin XuPublished in: Molecules (Basel, Switzerland) (2023)
Deer tendon, a deer processing byproduct, is an excellent protein source for the preparation of peptides for improving osteoporosis by its high protein content and high nutritional value. The optimal process of collagen acid extraction was implemented and the results showed that the acid concentration was 7%, the material-liquid ratio was 1:25, and the soaking time was 48 h. DTCHs could promote MC3T3-E1 cell proliferation and increase alkaline phosphatase activities in vitro. In addition, compared with the model group, the DTCHs treatment groups with an oral dosage of 350, 750, and 1500 mg/kg rat/day could significantly improve the shape, weight, bone mechanics, and alkaline phosphatase activities of tail-suspended mice. Bone microstructure and mineralization also recovered significantly in vivo. This result is expected to provide the structural and biological information for DTCHs-based functional foods.
Keyphrases
- bone mineral density
- postmenopausal women
- cell proliferation
- amino acid
- body composition
- randomized controlled trial
- protein protein
- soft tissue
- body mass index
- physical activity
- anterior cruciate ligament reconstruction
- white matter
- cell cycle
- oxidative stress
- weight loss
- healthcare
- bone loss
- rotator cuff
- bone regeneration
- type diabetes
- adipose tissue
- metabolic syndrome
- combination therapy
- pi k akt
- high resolution