A Highly Selective Chemical Probe for Activin Receptor-like Kinases ALK4 and ALK5.
Thomas HankeJong Fu WongBenedict-Tilmann BergerIsmahan AbdiLena Marie BergerRoberta TeschClaudia TredupAlex N BullockSusanne MüllerStefan KnappPublished in: ACS chemical biology (2020)
The transforming growth factor beta-receptor I/activin receptor-like kinase 5 (TGFBR1/ALK5) and its close homologue ALK4 are receptor protein kinases associated with the development of diverse diseases, including cancer, fibrosis, heart diseases, and dysfunctional immune response. Therefore, ALK4/5 are among the most studied kinases, and several inhibitors have been developed. However, current commercially available inhibitors either lack selectivity or have not been comprehensively characterized, limiting their value for studying ALK4/5 function in cellular systems. To this end, we report the characterization of the 2-oxo-imidazopyridine, TP-008, a potent chemical probe with dual activity for ALK4 and ALK5 as well as the development of a matching negative control compound. TP-008 has excellent cellular potency and strongly abrogates phosphorylation of the substrate SMAD2 (mothers against decapentaplegic homologue 2). Thus, this chemical probe offers an excellent tool for mechanistic studies on the ALK4/5 signaling pathway and the contribution of these targets to disease.
Keyphrases
- advanced non small cell lung cancer
- transforming growth factor
- immune response
- epithelial mesenchymal transition
- signaling pathway
- heart failure
- epidermal growth factor receptor
- squamous cell carcinoma
- binding protein
- oxidative stress
- small molecule
- protein kinase
- inflammatory response
- dendritic cells
- anti inflammatory
- single molecule
- induced apoptosis
- solid state