Effect of Surface Layer Proteins Derived from Paraprobiotic Kefir Lactic Acid Bacteria on Inflammation and High-Fat Diet-Induced Obesity.
Eseul KimHyeon Gyu LeeSanghoon HanKun-Ho SeoHyunsook KimPublished in: Journal of agricultural and food chemistry (2021)
The antiobesity action of nonviable probiotic lactic acid bacteria (PLAB) may be attributed to bacterial cellular components recognized by host cells. The anti-inflammation and antiobesity properties of surface layer proteins (SLPs) that are cellular components isolated from kefir PLAB were determined in macrophage RAW 264.7 cells and obese mice. Kefir SLPs significantly decreased secretion of IL-6 and production of NF-kB p65 protein by LPS-stimulated RAW 264.7 cells in a dose-response manner. C57BL/6J mice were fed a high-fat (HF) diet with oral administration of either saline (CON) or kefir SLPs for 6 weeks. SLPs significantly improved body weight gain and adipose tissue weight, plasma triglyceride concentrations, and insulin resistance. Profiling of adipocyte gene expression showed that the antiobesity effect was significantly related to the expression of genes associated with adipogenesis, autophagy, and inflammatory/immune response, and fatty acid oxidation. Taken together, SLPs are a novel bioactive component in kefir PLABs to target obesity and obesity-related disorders.
Keyphrases
- high fat diet induced
- insulin resistance
- adipose tissue
- weight gain
- lactic acid
- induced apoptosis
- oxidative stress
- high fat diet
- weight loss
- metabolic syndrome
- cell cycle arrest
- skeletal muscle
- gene expression
- body mass index
- signaling pathway
- immune response
- polycystic ovary syndrome
- type diabetes
- fatty acid
- birth weight
- endoplasmic reticulum stress
- cell death
- pi k akt
- heart failure
- inflammatory response
- toll like receptor
- dna methylation
- poor prognosis
- nitric oxide
- anti inflammatory
- dendritic cells
- body weight
- cell proliferation