Novel Hypocholesterolemic Peptides Derived from Silver Carp Muscle: The Modulatory Effects on Enterohepatic Cholesterol Metabolism In Vitro and In Vivo .
Kai WangLihua HanYuqing TanHui HongHongbing FanYongkang LuoPublished in: Journal of agricultural and food chemistry (2023)
This research aimed to investigate the effect of silver carp hydrolysates (SCHs) on hypercholesterolemia and enterohepatic cholesterol metabolism. Results showed that in vitro gastrointestinal digestion products of Alcalase-SCH (GID-Alcalase) exhibited the highest inhibitory activity of cholesterol absorption mainly through downregulating the expression of essential genes related to cholesterol transport in a Caco-2 monolayer. After being absorbed by the Caco-2 monolayer, GID-Alcalase increased the low-density lipoprotein (LDL) uptake in HepG2 cells by enhancing the protein level of the LDL receptor (LDLR). The in vivo experiment showed that long-term intervention of Alcalase-SCH ameliorated hypercholesterolemia in ApoE -/- mice fed with a Western diet (WD). After transepithelial transport, four novel peptides (TKY, LIL, FPK, and IAIM) were identified, and these peptides possessed dual hypocholesterolemic functions including inhibition of cholesterol absorption and promotion of peripheric LDL uptake. Our results indicated for the first time the potential of SCHs as functional food ingredients for the management of hypercholesterolemia.
Keyphrases
- low density lipoprotein
- amino acid
- gold nanoparticles
- randomized controlled trial
- physical activity
- poor prognosis
- skeletal muscle
- binding protein
- type diabetes
- cardiovascular disease
- genome wide
- weight loss
- high fat diet
- risk assessment
- metabolic syndrome
- adipose tissue
- insulin resistance
- transcription factor
- climate change
- small molecule
- cognitive decline
- high fat diet induced
- transition metal