Pan-SMARCA/PB1 Bromodomain Inhibitors and Their Role in Regulating Adipogenesis.
Marek WaniorFranziska PreussXiaomin NiAndreas KrämerSebastian MatheaTamara GöbelDavid HeidenreichSvenja SimonyiAstrid S KahntAndreas C JoergerStefan KnappPublished in: Journal of medicinal chemistry (2020)
Accessibility of the human genome is modulated by the ATP-driven SWI/SNF chromatin remodeling multiprotein complexes BAF (BRG1/BRM-associated factor) and PBAF (polybromo-associated BAF factor), which involves reading of acetylated histone tails by the bromodomain-containing proteins SMARCA2 (BRM), SMARCA4 (BRG1), and polybromo-1. Dysregulation of chromatin remodeling leads to aberrant cell proliferation and differentiation. Here, we have characterized a set of potent and cell-active bromodomain inhibitors with pan-selectivity for canonical family VIII bromodomains. Targeted SWI/SNF bromodomain inhibition blocked the expression of key genes during adipogenesis, including the transcription factors PPARγ and C/EBPα, and impaired the differentiation of 3T3-L1 murine fibroblasts into adipocytes. Our data highlight the role of SWI/SNF bromodomains in adipogenesis and provide a framework for the development of SWI/SNF bromodomain inhibitors for indirect targeting of key transcription factors regulating cell differentiation.
Keyphrases
- transcription factor
- genome wide
- cell proliferation
- high fat diet induced
- gene expression
- dna methylation
- dna damage
- poor prognosis
- endothelial cells
- genome wide identification
- cancer therapy
- adipose tissue
- dna binding
- single cell
- electronic health record
- working memory
- oxidative stress
- drug delivery
- metabolic syndrome
- machine learning
- fatty acid
- deep learning
- pluripotent stem cells
- bioinformatics analysis
- aqueous solution
- structural basis