Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging.
Ellen M QuardokusDiane C SaundersElizabeth McDonoughJohn W HickeyChristopher WerleinChristine SurrettePresha RajbhandariAnna Martinez CasalsHua TianLisa LoweryElizabeth K NeumannFrida BjörklundTaruna V NeelakantanJoshua CroteauAnne E WiblinJeremy FisherApril J LivengoodKaren G DowellJonathan C SilversteinJeffrey M SpragginsGloria S PryhuberGail DeutschFiona GintyGarry P NolanSimon MelovDanny D JonigkMichael A CaldwellIoannis S VlachosWerner MullerNils GehlenborgBrent R StockwellEmma LundbergMichael Paul SnyderRonald N GermainJeannie M CamarilloMichael P SnyderKaty BörnerAndrea J RadtkePublished in: Nature methods (2023)
Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.