Login / Signup

First Community-Wide, Comparative Cross-Linking Mass Spectrometry Study.

Claudio IacobucciChristine PiotrowskiRuedi AebersoldBruno C AmaralPhilip AndrewsKatja BernfurChristoph H BorchersNicolas I BrodieJames E BruceYong CaoStéphane ChaignepainJuan D ChavezStéphane ClaverolJuergen CoxTrisha DavisGianluca DegliespostiMeng-Qiu DongNufar EdingerCecilia EmanuelssonMarina GayMichael GötzeFrancisco Gomes-NetoFabio César GozzoCraig GutierrezCaroline HauptAlbert J R HeckFranz HerzogLan HuangMichael R HoopmannNir KalismanOleg KlykovZdeněk KukačkaFan LiuMichael J MacCossKarl MechtlerRavit MesikaRobert L MoritzNagarjuna NagarajVictor NesatiAna G C Neves-FerreiraRobert NinnisPetr NovákFrancis J O'ReillyMatthias PelzingEvgeniy PetrotchenkoLolita PiersimoniManolo PlasenciaTara Louise PukalaKasper Dyrberg RandJuri RappsilberDana ReichmannCarolin SailerChris P SarnowskiRichard A ScheltemaCarla SchmidtDavid C SchriemerYi ShiJ Mark SkehelMoriya SlavinFrank SobottVictor Solis-MezarinoHeike StephanowitzFlorian StengelChristian E StiegerEsben TrabjergMichael TrnkaMarta VilasecaRosa VinerYufei XiangSule YilmazAlex ZelterDaniel ZiemianowiczAlexander LeitnerAndrea Sinz
Published in: Analytical chemistry (2019)
The number of publications in the field of chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and to probe protein-protein interactions has increased during the last years. As the technique is now becoming routine for in vitro and in vivo applications in proteomics and structural biology there is a pressing need to define protocols as well as data analysis and reporting formats. Such consensus formats should become accepted in the field and be shown to lead to reproducible results. This first, community-based harmonization study on XL-MS is based on the results of 32 groups participating worldwide. The aim of this paper is to summarize the status quo of XL-MS and to compare and evaluate existing cross-linking strategies. Our study therefore builds the framework for establishing best practice guidelines to conduct cross-linking experiments, perform data analysis, and define reporting formats with the ultimate goal of assisting scientists to generate accurate and reproducible XL-MS results.
Keyphrases
  • mass spectrometry
  • data analysis
  • multiple sclerosis
  • liquid chromatography
  • healthcare
  • clinical practice
  • high performance liquid chromatography
  • emergency department
  • mental health