Florian Hollfelder

ORCID

Publication Activity (10 Years)

Years Active: 2015-2024
Publications (10 Years): 85

Top Venues

Nature communications

Analytical chemistry

Journal of the American Chemical Society

Chemical reviews

University of Cambridge

This page only lists publications with an associated author ORCID identifier.

Affiliation History

University of Cambridge Oct 01 2001

United Kingdom

Professor of Chemical and Synthetic Biology :: Biochemistry

Publications

Maximilian Gantz, Simon V Mathis, Friederike E H Nintzel, Pietro Lió, Florian Hollfelder
On synergy between ultrahigh throughput screening and machine learning in biocatalyst engineering. Faraday discussions (2024)
J David Schnettler, Michael S Wang, Maximilian Gantz, H Adrian Bunzel, Christina Karas, Florian Hollfelder, Michael H Hecht
Selection of a promiscuous minimalist cAMP phosphodiesterase from a library of de novo designed proteins. Nature chemistry 16 (7) (2024)
J David Schnettler, Michael S Wang, Maximilian Gantz, H Adrian Bunzel, Christina Karas, Florian Hollfelder, Michael H Hecht
Selection of a promiscuous minimalist cAMP phosphodiesterase from a library of de novo designed proteins. Nature chemistry 16 (7) (2024)
Carlos Acevedo-Rocha, Łukasz Berlicki, Uwe T Bornscheuer, Dominic J Campopiano, Pimchai Chaiyen, Janko Čivić, Zhiqi Cong, Friedrich Johannes Ehinger, Sabine Flitsch, Artur Góra, Marko Hanzevacki, Jeremy N Harvey, Donald Hilvert, Florian Hollfelder, Amanda G Jarvis, Bruce R Lichtenstein, Stefan Lutz, Thomas Malcomson, E Neil G Marsh, Neil R McFarlane, Alexander McKenzie, Adrian Mulholland, Sílvia Osuna, Joelle N Pelletier, Agata Raczyńska, Gerard Roelfes, Lubomír Rulíšek, Peter Stockinger, Nicholas Turner, Francesca Valetti, Marc Van der Kamp, Mikael Widersten, Cathleen Zeymer
Enzyme evolution, engineering and design: mechanism and dynamics: general discussion. Faraday discussions (2024)
Maximilian Gantz, Simon V Mathis, Friederike E H Nintzel, Pietro Lió, Florian Hollfelder
On synergy between ultrahigh throughput screening and machine learning in biocatalyst engineering. Faraday discussions (2024)
Katrin Fischer, Aleksei Lulla, Tsz Y So, Pehuén Pereyra-Gerber, Matthew I J Raybould, Timo N Kohler, Juan Carlos Yam-Puc, Tomasz S Kaminski, Robert Hughes, Gwendolyn L Pyeatt, Florian Leiss-Maier, Paul D Brear, Nicholas J Matheson, Charlotte M Deane, Marko Hyvönen, James E D Thaventhiran, Florian Hollfelder
Rapid discovery of monoclonal antibodies by microfluidics-enabled FACS of single pathogen-specific antibody-secreting cells. Nature biotechnology (2024)
J David Schnettler, Michael S Wang, Maximilian Gantz, H Adrian Bunzel, Christina Karas, Florian Hollfelder, Michael H Hecht
Selection of a promiscuous minimalist cAMP phosphodiesterase from a library of de novo designed proteins. Nature chemistry (2024)
J David Schnettler, Michael S Wang, Maximilian Gantz, H Adrian Bunzel, Christina Karas, Florian Hollfelder, Michael H Hecht
Selection of a promiscuous minimalist cAMP phosphodiesterase from a library of de novo designed proteins. Nature chemistry 16 (7) (2024)
Maximilian Gantz, Simon V Mathis, Friederike E H Nintzel, Pietro Lió, Florian Hollfelder
On synergy between ultrahigh throughput screening and machine learning in biocatalyst engineering. Faraday discussions (2024)
Remkes A Scheele, Yanik Weber, Friederike E H Nintzel, Michael Herger, Tomasz S Kaminski, Florian Hollfelder
Ultrahigh Throughput Evolution of Tryptophan Synthase in Droplets via an Aptamer Sensor. ACS catalysis 14 (8) (2024)
Remkes A Scheele, Yanik Weber, Friederike E H Nintzel, Michael Herger, Tomasz S Kaminski, Florian Hollfelder
Ultrahigh Throughput Evolution of Tryptophan Synthase in Droplets via an Aptamer Sensor. ACS catalysis 14 (8) (2024)
Susanna Zamolo, Elena Zakharova, Lise Boursinhac, Florian Hollfelder, Tamis Darbre, Jean-Louis Reymond
Peptide dendrimers transfecting CRISPR/Cas9 plasmid DNA: optimization and mechanism. RSC chemical biology 5 (9) (2024)
Juan Carlos Yam-Puc, Zhaleh Hosseini, Emily C Horner, Pehuén Pereyra Gerber, Nonantzin Beristain-Covarrubias, Robert Hughes, Aleksei Lulla, Maria Rust, Rebecca Boston, Magda Ali, Katrin Fischer, Edward Simmons-Rosello, Martin J O'Reilly, Harry Robson, Lucy H Booth, Lakmini Kahanawita, Andrea Correa-Noguera, David Favara, Lourdes Ceron-Gutierrez, Baerbel Keller, Andrew Craxton, Georgina S F Anderson, Xiao-Ming Sun, Anne Elmer, Caroline Saunders, Areti Bermperi, Sherly Jose, Nathalie Kingston, Thomas E Mulroney, Lucia P G Piñon, null null, Michael A Chapman, Sofia Grigoriadou, Marion MacFarlane, Anne E Willis, Kiran R Patil, Sarah Spencer, Emily Staples, Klaus Warnatz, Matthew S Buckland, Florian Hollfelder, Marko Hyvönen, Rainer Doffinger, Christine Parkinson, Sara Lear, Nicholas J Matheson, James E D Thaventhiran
Age-associated B cells predict impaired humoral immunity after COVID-19 vaccination in patients receiving immune checkpoint blockade. Nature communications 14 (1) (2023)
Philipp Knyphausen, Mariana Rangel Pereira, Paul D Brear, Marko Hyvönen, Lutz Jermutus, Florian Hollfelder
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture. Nature communications 14 (1) (2023)
Maximilian Gantz, Stefanie Neun, Elliot J Medcalf, Liisa D van Vliet, Florian Hollfelder
Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments. Chemical reviews 123 (9) (2023)
Juan Carlos Yam-Puc, Zhaleh Hosseini, Emily C Horner, Pehuén Pereyra Gerber, Nonantzin Beristain-Covarrubias, Robert Hughes, Aleksei Lulla, Maria Rust, Rebecca Boston, Magda Ali, Katrin Fischer, Edward Simmons-Rosello, Martin J O'Reilly, Harry Robson, Lucy H Booth, Lakmini Kahanawita, Andrea Correa-Noguera, David Favara, Lourdes Ceron-Gutierrez, Baerbel Keller, Andrew Craxton, Georgina S F Anderson, Xiao-Ming Sun, Anne Elmer, Caroline Saunders, Areti Bermperi, Sherly Jose, Nathalie Kingston, Thomas E Mulroney, Lucia P G Piñon, null null, Michael A Chapman, Sofia Grigoriadou, Marion MacFarlane, Anne E Willis, Kiran R Patil, Sarah Spencer, Emily Staples, Klaus Warnatz, Matthew S Buckland, Florian Hollfelder, Marko Hyvönen, Rainer Doffinger, Christine Parkinson, Sara Lear, Nicholas J Matheson, James E D Thaventhiran
Age-associated B cells predict impaired humoral immunity after COVID-19 vaccination in patients receiving immune checkpoint blockade. Nature communications 14 (1) (2023)
Elliot J Medcalf, Maximilian Gantz, Tomasz S Kaminski, Florian Hollfelder
Ultra-High-Throughput Absorbance-Activated Droplet Sorting for Enzyme Screening at Kilohertz Frequencies. Analytical chemistry 95 (10) (2023)
Juan Carlos Yam-Puc, Zhaleh Hosseini, Emily C Horner, Pehuén Pereyra Gerber, Nonantzin Beristain-Covarrubias, Robert Hughes, Aleksei Lulla, Maria Rust, Rebecca Boston, Magda Ali, Katrin Fischer, Edward Simmons-Rosello, Martin J O'Reilly, Harry Robson, Lucy H Booth, Lakmini Kahanawita, Andrea Correa-Noguera, David Favara, Lourdes Ceron-Gutierrez, Baerbel Keller, Andrew Craxton, Georgina S F Anderson, Xiao-Ming Sun, Anne Elmer, Caroline Saunders, Areti Bermperi, Sherly Jose, Nathalie Kingston, Thomas E Mulroney, Lucia P G Piñon, null null, Michael A Chapman, Sofia Grigoriadou, Marion MacFarlane, Anne E Willis, Kiran R Patil, Sarah Spencer, Emily Staples, Klaus Warnatz, Matthew S Buckland, Florian Hollfelder, Marko Hyvönen, Rainer Doffinger, Christine Parkinson, Sara Lear, Nicholas J Matheson, James E D Thaventhiran
Age-associated B cells predict impaired humoral immunity after COVID-19 vaccination in patients receiving immune checkpoint blockade. Nature communications 14 (1) (2023)
Elliot J Medcalf, Maximilian Gantz, Tomasz S Kaminski, Florian Hollfelder
Ultra-High-Throughput Absorbance-Activated Droplet Sorting for Enzyme Screening at Kilohertz Frequencies. Analytical chemistry 95 (10) (2023)
Maximilian Gantz, Stefanie Neun, Elliot J Medcalf, Liisa D van Vliet, Florian Hollfelder
Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments. Chemical reviews 123 (9) (2023)
Elliot J Medcalf, Maximilian Gantz, Tomasz S Kaminski, Florian Hollfelder
Ultra-High-Throughput Absorbance-Activated Droplet Sorting for Enzyme Screening at Kilohertz Frequencies. Analytical chemistry 95 (10) (2023)
Elliot J Medcalf, Maximilian Gantz, Tomasz S Kaminski, Florian Hollfelder
Ultra-High-Throughput Absorbance-Activated Droplet Sorting for Enzyme Screening at Kilohertz Frequencies. Analytical chemistry (2023)
Philipp Knyphausen, Mariana Rangel Pereira, Paul D Brear, Marko Hyvönen, Lutz Jermutus, Florian Hollfelder
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture. Nature communications 14 (1) (2023)
Elliot J Medcalf, Maximilian Gantz, Tomasz S Kaminski, Florian Hollfelder
Ultra-High-Throughput Absorbance-Activated Droplet Sorting for Enzyme Screening at Kilohertz Frequencies. Analytical chemistry 95 (10) (2023)
Maximilian Gantz, Stefanie Neun, Elliot J Medcalf, Liisa D van Vliet, Florian Hollfelder
Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments. Chemical reviews 123 (9) (2023)
Timo N Kohler, Joachim De Jonghe, Anna L Ellermann, Ayaka Yanagida, Michael Herger, Erin M Slatery, Antonia Weberling, Clara Munger, Katrin Fischer, Carla Mulas, Alex Winkel, Connor Ross, Sophie Bergmann, Kristian Franze, Kevin J Chalut, Jennifer Nichols, Thorsten E Boroviak, Florian Hollfelder
Plakoglobin is a mechanoresponsive regulator of naive pluripotency. Nature communications 14 (1) (2023)
Joachim De Jonghe, Tomasz S Kaminski, David B Morse, Marcin Tabaka, Anna L Ellermann, Timo N Kohler, Gianluca Amadei, Charlotte E Handford, Gregory M Findlay, Magdalena Zernicka Goetz, Sarah A Teichmann, Florian Hollfelder
spinDrop: a droplet microfluidic platform to maximise single-cell sequencing information content. Nature communications 14 (1) (2023)
Maximilian Gantz, Stefanie Neun, Elliot J Medcalf, Liisa D van Vliet, Florian Hollfelder
Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments. Chemical reviews 123 (9) (2023)
Juan Carlos Yam-Puc, Zhaleh Hosseini, Emily C Horner, Pehuén Pereyra Gerber, Nonantzin Beristain-Covarrubias, Robert Hughes, Aleksei Lulla, Maria Rust, Rebecca Boston, Magda Ali, Katrin Fischer, Edward Simmons-Rosello, Martin J O'Reilly, Harry Robson, Lucy H Booth, Lakmini Kahanawita, Andrea Correa-Noguera, David Favara, Lourdes Ceron-Gutierrez, Baerbel Keller, Andrew Craxton, Georgina S F Anderson, Xiao-Ming Sun, Anne Elmer, Caroline Saunders, Areti Bermperi, Sherly Jose, Nathalie Kingston, Thomas E Mulroney, Lucia P G Piñon, null null, Michael A Chapman, Sofia Grigoriadou, Marion MacFarlane, Anne E Willis, Kiran R Patil, Sarah Spencer, Emily Staples, Klaus Warnatz, Matthew S Buckland, Florian Hollfelder, Marko Hyvönen, Rainer Doffinger, Christine Parkinson, Sara Lear, Nicholas J Matheson, James E D Thaventhiran
Age-associated B cells predict impaired humoral immunity after COVID-19 vaccination in patients receiving immune checkpoint blockade. Nature communications 14 (1) (2023)
Maximilian Gantz, Stefanie Neun, Elliot J Medcalf, Liisa D van Vliet, Florian Hollfelder
Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments. Chemical reviews 123 (9) (2023)
Subhajit Bhattacharjee, Chengzhi Guo, Erwin Lam, Josephin M Holstein, Mariana Rangel Pereira, Christian M Pichler, Chanon Pornrungroj, Motiar Rahaman, Taylor Uekert, Florian Hollfelder, Erwin Reisner
Chemoenzymatic Photoreforming: A Sustainable Approach for Solar Fuel Generation from Plastic Feedstocks. Journal of the American Chemical Society (2023)
Maximilian Gantz, Stefanie Neun, Elliot J Medcalf, Liisa D van Vliet, Florian Hollfelder
Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments. Chemical reviews 123 (9) (2023)
Maximilian Gantz, Stefanie Neun, Elliot J Medcalf, Liisa D van Vliet, Florian Hollfelder
Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments. Chemical reviews (2023)
Philipp Knyphausen, Mariana Rangel Pereira, Paul D Brear, Marko Hyvönen, Lutz Jermutus, Florian Hollfelder
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture. Nature communications 14 (1) (2023)
Min Bao, Jake Cornwall-Scoones, Estefania Sanchez-Vasquez, Andy L Cox, Dong-Yuan Chen, Joachim De Jonghe, Shahriar Shadkhoo, Florian Hollfelder, Matt Thomson, David M Glover, Magdalena Zernicka Goetz
Author Correction: Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension. Nature cell biology (2023)
Philipp Knyphausen, Mariana Rangel Pereira, Paul D Brear, Marko Hyvönen, Lutz Jermutus, Florian Hollfelder
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture. Nature communications 14 (1) (2023)
Philipp Knyphausen, Mariana Rangel Pereira, Paul D Brear, Marko Hyvönen, Lutz Jermutus, Florian Hollfelder
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture. Nature communications 14 (1) (2023)
Simon Ladeveze, Paul Jannis Zurek, Tomasz S Kaminski, Stephane Emond, Florian Hollfelder
Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets. ACS catalysis 13 (15) (2023)
Louis F L Wilson, Stefanie Neun, Li Yu, Theodora Tryfona, Katherine Stott, Florian Hollfelder, Paul Dupree
The biosynthesis, degradation, and function of cell wall β-xylosylated xyloglucan mirrors that of arabinoxyloglucan. The New phytologist (2023)
Philipp Knyphausen, Mariana Rangel Pereira, Paul D Brear, Marko Hyvönen, Lutz Jermutus, Florian Hollfelder
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture. Nature communications 14 (1) (2023)
Maximilian Gantz, Stefanie Neun, Elliot J Medcalf, Liisa D van Vliet, Florian Hollfelder
Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments. Chemical reviews (2023)
J David Schnettler, Oskar James Klein, Tomasz S Kaminski, Pierre-Yves Colin, Florian Hollfelder
Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase. Journal of the American Chemical Society 145 (2) (2022)
Stefanie Neun, Paul D Brear, Eleanor Campbell, Theodora Tryfona, Kamel El Omari, Armin Wagner, Paul Dupree, Marko Hyvönen, Florian Hollfelder
Functional metagenomic screening identifies an unexpected β-glucuronidase. Nature chemical biology 18 (10) (2022)
J David Schnettler, Oskar James Klein, Tomasz S Kaminski, Pierre-Yves Colin, Florian Hollfelder
Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase. Journal of the American Chemical Society 145 (2) (2022)
Clara Munger, Timo N Kohler, Erin M Slatery, Anna L Ellermann, Sophie Bergmann, Christopher A Penfold, Ioakeim Ampartzidis, Yutong Chen, Florian Hollfelder, Thorsten E Boroviak
Microgel culture and spatial identity mapping elucidate the signalling requirements for primate epiblast and amnion formation. Development (Cambridge, England) 149 (20) (2022)
Stefanie Neun, Liisa van Vliet, Florian Hollfelder, Fabrice Gielen
High-Throughput Steady-State Enzyme Kinetics Measured in a Parallel Droplet Generation and Absorbance Detection Platform. Analytical chemistry 94 (48) (2022)
Fredrik Salmen, Joachim De Jonghe, Tomasz S Kaminski, Anna Alemany, Guillermo E Parada, Joe Verity-Legg, Ayaka Yanagida, Timo N Kohler, Nicholas Battich, Floris van den Brekel, Anna L Ellermann, Alfonso Martinez Arias, Jennifer Nichols, Martin Hemberg, Florian Hollfelder, Alexander van Oudernaarden
High-throughput total RNA sequencing in single cells using VASA-seq. Nature biotechnology (2022)
J David Schnettler, Oskar James Klein, Tomasz S Kaminski, Pierre-Yves Colin, Florian Hollfelder
Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase. Journal of the American Chemical Society 145 (2) (2022)
Esther S Richter, Andreas Link, John S McGrath, Raymond W Sparrow, Maximilian Gantz, Elliot J Medcalf, Florian Hollfelder, Thomas Franke
Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance. Lab on a chip (2022)
Min Bao, Jake Cornwall-Scoones, Estefania Sanchez-Vasquez, Dong-Yuan Chen, Joachim De Jonghe, Shahriar Shadkhoo, Florian Hollfelder, Matt Thomson, David M Glover, Magdalena Zernicka Goetz
Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension. Nature cell biology 24 (9) (2022)
J David Schnettler, Oskar James Klein, Tomasz S Kaminski, Pierre-Yves Colin, Florian Hollfelder
Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase. Journal of the American Chemical Society 145 (2) (2022)
J David Schnettler, Oskar James Klein, Tomasz S Kaminski, Pierre-Yves Colin, Florian Hollfelder
Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase. Journal of the American Chemical Society (2022)
Clara Munger, Timo N Kohler, Erin Slatery, Anna L Ellermann, Sophie Bergmann, Christopher Penfold, Ioakeim Ampartzidis, Yutong Chen, Florian Hollfelder, Thorsten E Boroviak
Microgel culture and spatial identity mapping elucidate the signalling requirements for primate epiblast and amnion formation. Development (Cambridge, England) (2022)
Thomas Fryer, Joel David Rogers, Christopher Mellor, Timo N Kohler, Ralph Minter, Florian Hollfelder
Gigavalent Display of Proteins on Monodisperse Polyacrylamide Hydrogels as a Versatile Modular Platform for Functional Assays and Protein Engineering. ACS central science 8 (8) (2022)
Remkes A Scheele, Laurens H Lindenburg, Maya Petek, Markus Schober, Kevin N Dalby, Florian Hollfelder
Droplet-based screening of phosphate transfer catalysis reveals how epistasis shapes MAP kinase interactions with substrates. Nature communications 13 (1) (2022)
J David Schnettler, Oskar James Klein, Tomasz S Kaminski, Pierre-Yves Colin, Florian Hollfelder
Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase. Journal of the American Chemical Society 145 (2) (2022)
J David Schnettler, Oskar James Klein, Tomasz S Kaminski, Pierre-Yves Colin, Florian Hollfelder
Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase. Journal of the American Chemical Society 145 (2) (2022)
Gianluca Amadei, Charlotte E Handford, Chengxiang Qiu, Joachim De Jonghe, Hannah Greenfeld, Martin Tran, Beth K Martin, Dong-Yuan Chen, Alejandro Aguilera-Castrejon, Jacob H Hanna, Michael B Elowitz, Florian Hollfelder, Jay Shendure, David M Glover, Magdalena Zernicka Goetz
Embryo model completes gastrulation to neurulation and organogenesis. Nature 610 (7930) (2022)
Godwin A Aleku, Gabriel R Titchiner, George W Roberts, Sasha R Derrington, James R Marshall, Florian Hollfelder, Nicholas J Turner, David Leys
Enzymatic N -Allylation of Primary and Secondary Amines Using Renewable Cinnamic Acids Enabled by Bacterial Reductive Aminases. ACS sustainable chemistry & engineering 10 (20) (2022)
Sophie Bergmann, Christopher A Penfold, Erin M Slatery, Dylan Siriwardena, Charis Drummer, Stephen J Clark, Stanley E Strawbridge, Keiko Kishimoto, Alice Vickers, Mukul Tewary, Timo N Kohler, Florian Hollfelder, Juan I Garaycoechea, Erika Sasaki, Rüdiger Behr, Thorsten E Boroviak
Spatial profiling of early primate gastrulation in utero. Nature 609 (7925) (2022)
Laurens Lindenburg, Florian Hollfelder
"NAD-display": Ultrahigh-Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry. Angewandte Chemie (International ed. in English) 60 (16) (2021)
Tuomas Huovinen, Laurens Lindenburg, Ralph Minter, Florian Hollfelder
Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent Capture on Suspension Bead Arrays. Analytical chemistry 93 (4) (2021)
Andrea Schenkmayerova, Gaspar P Pinto, Martin Toul, Martin Marek, Lenka Hernychova, Joan Planas-Iglesias, Veronika Daniel Liskova, Daniel Pluskal, Michal Vasina, Stephane Emond, Mark Dörr, Radka Chaloupkova, David Bednar, Zbynek Prokop, Florian Hollfelder, Uwe T Bornscheuer, Jiří Damborský
Engineering the protein dynamics of an ancestral luciferase. Nature communications 12 (1) (2021)
Laurens H Lindenburg, Teodors Pantelejevs, Fabrice Gielen, Pedro Zuazua-Villar, Maren Butz, Eric Rees, Clemens F Kaminski, Jessica A Downs, Marko Hyvönen, Florian Hollfelder
Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats. Proceedings of the National Academy of Sciences of the United States of America 118 (46) (2021)
Miriam Klaus, Paul Jannis Zurek, Tomasz S Kaminski, Ahir Pushpanath, Katharina Neufeld, Florian Hollfelder
Ultrahigh-Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay. Chembiochem : a European journal of chemical biology 22 (23) (2021)
George S Biggs, Oskar James Klein, Sarah L Maslen, J Mark Skehel, Trevor J Rutherford, Stefan M V Freund, Florian Hollfelder, Sally R Boss, Paul D Barker
Controlled Ligand Exchange Between Ruthenium Organometallic Cofactor Precursors and a Naïve Protein Scaffold Generates Artificial Metalloenzymes Catalysing Transfer Hydrogenation. Angewandte Chemie (International ed. in English) 60 (19) (2021)
Josephin M Holstein, Christian Gylstorff, Florian Hollfelder
Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput. ACS synthetic biology 10 (2) (2021)
Che Fai Alex Wong, Liisa van Vliet, Swapnil Vilas Bhujbal, Chengzhi Guo, Marit Sletmoen, Bjo Rn Torger Stokke, Florian Hollfelder, Rahmi Lale
A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution. ACS synthetic biology 10 (8) (2021)
Paul Jannis Zurek, Philipp Knyphausen, Katharina Neufeld, Ahir Pushpanath, Florian Hollfelder
UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution. Nature communications 11 (1) (2020)
Stephane Emond, Maya Petek, Emily J Kay, Brennen Heames, Sean R A Devenish, Nobuhiko Tokuriki, Florian Hollfelder
Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis. Nature communications 11 (1) (2020)
Kalliopi Skamaki, Stephane Emond, Matthieu Chodorge, John Andrews, D Gareth Rees, Daniel Cannon, Bojana Popovic, Andrew Buchanan, Ralph R Minter, Florian Hollfelder
In vitro evolution of antibody affinity via insertional scanning mutagenesis of an entire antibody variable region. Proceedings of the National Academy of Sciences of the United States of America 117 (44) (2020)
Syue-Ting Kuo, Ruey-Lin Jahn, Yuan-Ju Cheng, Yi-Lan Chen, Yun-Ju Lee, Florian Hollfelder, Jin-Der Wen, Hsin-Hung David Chou
Global fitness landscapes of the Shine-Dalgarno sequence. Genome research 30 (5) (2020)
Bert van Loo, Ryan Berry, Usa Boonyuen, Mark F Mohamed, Marko Golicnik, Alvan C Hengge, Florian Hollfelder
Transition-State Interactions in a Promiscuous Enzyme: Sulfate and Phosphate Monoester Hydrolysis by Pseudomonas aeruginosa Arylsulfatase. Biochemistry 58 (10) (2019)
Tomas Buryska, Michal Vasina, Fabrice Gielen, Pavel Vanacek, Liisa van Vliet, Jan Jezek, Zdenek Pilat, Pavel Zemanek, Jiří Damborský, Florian Hollfelder, Zbynek Prokop
Controlled Oil/Water Partitioning of Hydrophobic Substrates Extending the Bioanalytical Applications of Droplet-Based Microfluidics. Analytical chemistry 91 (15) (2019)
Bert van Loo, Magdalena Heberlein, Philip Mair, Anastasia Zinchenko, Jan Schüürmann, Bernard D G Eenink, Josephin M Holstein, Carina Dilkaute, Joachim Jose, Florian Hollfelder, Erich Bornberg-Bauer
High-Throughput, Lysis-Free Screening for Sulfatase Activity Using Escherichia coli Autodisplay in Microdroplets. ACS synthetic biology 8 (12) (2019)
Charlotte M Miton, Stefanie Jonas, Gerhard Fischer, Fernanda Duarte, Mark F Mohamed, Bert van Loo, Bálint Kintses, Shina Caroline Lynn Kamerlin, Nobuhiko Tokuriki, Marko Hyvönen, Florian Hollfelder
Evolutionary repurposing of a sulfatase: A new Michaelis complex leads to efficient transition state charge offset. Proceedings of the National Academy of Sciences of the United States of America 115 (31) (2018)
Hans Kleine-Brüggeney, Liisa D van Vliet, Carla Mulas, Fabrice Gielen, Chibeza C Agley, José C R Silva, Austin Smith, Kevin Chalut, Florian Hollfelder
Long-Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation. Small (Weinheim an der Bergstrasse, Germany) 15 (5) (2018)
Elizabeth Bilsland, Liisa van Vliet, Kevin Williams, Jack Feltham, Marta P Carrasco, Wesley L Fotoran, Eliana F G Cubillos, Gerhard Wunderlich, Morten Grøtli, Florian Hollfelder, Victoria Jackson, Ross D King, Stephen G Oliver
Plasmodium dihydrofolate reductase is a second enzyme target for the antimalarial action of triclosan. Scientific reports 8 (1) (2018)
Bert van Loo, Christopher D Bayer, Gerhard Fischer, Stefanie Jonas, Eugene Valkov, Mark F Mohamed, Anastassia Vorobieva, Celine Dutruel, Marko Hyvönen, Florian Hollfelder
Balancing Specificity and Promiscuity in Enzyme Evolution: Multidimensional Activity Transitions in the Alkaline Phosphatase Superfamily. Journal of the American Chemical Society 141 (1) (2018)
Christopher D Bayer, Bert van Loo, Florian Hollfelder
Specificity Effects of Amino Acid Substitutions in Promiscuous Hydrolases: Context-Dependence of Catalytic Residue Contributions to Local Fitness Landscapes in Nearby Sequence Space. Chembiochem : a European journal of chemical biology 18 (11) (2017)
Fabrice Gielen, Maren Butz, Eric J Rees, Miklos Erdelyi, Tommaso Moschetti, Marko Hyvönen, Joshua B Edel, Clemens F Kaminski, Florian Hollfelder
Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets. Analytical chemistry 89 (2) (2017)
Albert Kwok, Gabriela A Eggimann, Marc Heitz, Jean-Louis Reymond, Florian Hollfelder, Tamis Darbre
Efficient Transfection of siRNA by Peptide Dendrimer-Lipid Conjugates. Chembiochem : a European journal of chemical biology 17 (23) (2016)
Miriam Kaltenbach, Stephane Emond, Florian Hollfelder, Nobuhiko Tokuriki
Functional Trade-Offs in Promiscuous Enzymes Cannot Be Explained by Intrinsic Mutational Robustness of the Native Activity. PLoS genetics 12 (10) (2016)
Eleanor Campbell, Miriam Kaltenbach, Galen J Correy, Paul D Carr, Benjamin T Porebski, Emma K Livingstone, Livnat Afriat-Jurnou, Ashley M Buckle, Martin Weik, Florian Hollfelder, Nobuhiko Tokuriki, Colin J Jackson
The role of protein dynamics in the evolution of new enzyme function. Nature chemical biology 12 (11) (2016)
Fabrice Gielen, Raphaelle Hours, Stephane Emond, Martin Fischlechner, Ursula Schell, Florian Hollfelder
Ultrahigh-throughput-directed enzyme evolution by absorbance-activated droplet sorting (AADS). Proceedings of the National Academy of Sciences of the United States of America 113 (47) (2016)
Pierre-Yves Colin, Balint Kintses, Fabrice Gielen, Charlotte M Miton, Gerhard Fischer, Mark F Mohamed, Marko Hyvönen, Diego P Morgavi, Dick B Janssen, Florian Hollfelder
Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics. Nature communications 6 (2015)

Florian Hollfelder.css-eqlu8i{font-size:small;padding-left:4px;}

Publication Activity (10 Years)

Top Topics

Top Venues

Florian Hollfelder